Profile

Rahul Jamdade, PhD

Survey No. 36/5/1, Plot No. 2, Vikasnagar • Satara-415003 • Maharashtra • India.
IND (91) 9766816678 • UAE (971) 554672949. 
Email: rajamdade@gmail.com


HIGHLIGHTS


SUMMARY OF QUALIFICATIONS

Self-motivated and diligent professional with over 5+ years of post-doctoral research experience in molecular biology. Exceptional expertise with an extensive research background in molecular biology techniques, bioinformatics and genomics. Demonstrated DNA barcoding for species identification by generating 2000+ multi-locus DNA barcodes with 10+ publications in leading journals. Highly organized, collaborative professional and strategic planner with project management skills. Ability to work accurately, independently as well as part of a team.


EDUCATION

Ph.D. in Zoology (2009 – 2014), Paul Hebert Center for DNA Barcoding and Biodiversity Studies, Dr. Babasaheb Ambedkar Marathwada University, Maharashtra, India.


Master of Science (2006 – 2008), Shivaji University, Maharashtra, India.

Bachelor of Science (2003 – 2006), Shivaji University, Maharashtra, India.

RESEARCH EXPERIENCE
 

Environment and Protected Areas Authority, United Arab Emirates (UAE) 

Senior Research Specialist (Post-doctoral Researcher) (May 2017 – July 2022)

Research summary:
  • Generating the multi-locus DNA barcode reference library (1000+ sequences) for the plants (250+ species) of the United Arab Emirates
  • DNA barcoding of the medicinal plant species (250+ sequences, to assist pharmacovigilance across the UAE. 
  • Resolving cryptic species via multi-locus marker-based delimitation assisted by a supervised machine learning approach.
  • Creating a framework for the DNA bank.
  • Genome-wide identification of mitogen-activated protein (MAP) kinase gene family in Castor.
  • Demonstrating the AI-based machine learning approach for evaluating the Arabian plant barcodes and integrating the models in the DNA barcoding workflow for resolving the UAE plant species. 

Roles and Responsibilities:
  • Developing or strengthening Laboratory Infrastructure. 
  • Designing and implementing the research projects.
  • Monitoring research activities.
  • Mentoring the research assistants. 
  • Effective communication
  • Acquisition of results and data analysis 
  • Scientific writing and publication.

UGC Major Research Project at Rani Laxmibai Mahavidyala, Jalgaon, India. 

Junior Research Fellow (Aug 2011 – Nov 2012)

Project entitled: Molecular approach in understanding the genetic diversity of Mosquitoes from Maharashtra by using COI gene.

Research summary: Collection, preservation, DNA extraction, amplification, and sequencing of the mitochondrial CO1 gene. Sequence alignment and assembly and related bioinformatics analysis, further reporting results to the principal investigator, and preparing the annual report.


TEACHING EXPERIENCE

Yashvantrao Chavan Institute of Science, Satara, India.

Assistant professor (Contractual) (July 2014 – Feb 2017)

Conducting lectures, practicals, annual projects, and field trips for Bachelor (Zoology – Fisheries) and Masters (Zoology – Cell biology – Bioinformatics). 


SKILLS AND TECHNICAL ACQUISITION

Technique acquisition
  • DNA Sequencer (Genetic Analyzer 3130) sequencing, calibration, and independent handling.
  • Primer designing and synthesis (Mermaid Oligo Synthesizer). 
  • PCR amplification, troubleshooting, and formulation. 
  • Genome-wide identification of genes and expression analysis.
 Computer proficiency
  • DNA sequence alignment and assembly using Geneious Prime, Codon code Aligner, and MEGA5,
  • DNA Sequence analysis using Genious, Arlequin, and DNASP. 
  • AI-based Machine learning technique using WEKA for classification of species (DNA Barcodes)
  • Primer designing (conventional and qPCR with probes) using; Primer 3, IDT Primer quest, & Oligo clac. 
  • Network software for Detecting structure from genetic data.
  • Recognition of complimentary genes using Genome repositories.
  • Recognition of relevant domains using HMM profiling.
  • Signature MOTIF search using ScanProsite.
  • Elimination of redundant sequences using CD-HIT and EMBOSS.
Oral and written skills
  • Effective verbal communication and interpersonal skills
  • Scientific/academic writing and communication
  • Speaking fluently English, Hindi, Marathi
  • Writing English, Hindi, Marathi

GRANTS

International Travel Grant, Government of Sharjah, UAE, for “8th International Barcode of Life Conference”, Norway (2019).


RESEARCH PUBLICATIONS

  1. Jamdade, R., Al Shaer, K., Al Sallani, M., Al Harthi, E., Mahmoud, T., Gairola, S., Shabana, H., (2022). Multilocus Marker-based Delimitation of Salicornia persica and its Population Discrimination Assisted by Supervised Machine Learning Approach. PLoS ONE. (Accepted).
  2. Jamdade, R., Mosa, K. A., El-Keblawy, A., Shaer, K. Al, Harthi, E. Al, Sallani, M. Al, … Mahmoud, T. (2022). DNA Barcodes for Accurate Identification of Selected Medicinal Plants (Caryophyllales): Toward Barcoding Flowering Plants of the United Arab Emirates. Diversity 2022, Vol. 14, Page 262, 14(4), 262. https://doi.org/10.3390/D14040262
  3. Jamdade, R., Upadhyay, M., Al Shaer, K., Al Harthi, E., Al Sallani, M., Al Jasmi, M., & Al Ketbi, A. (2021). Evaluation of Arabian Vascular Plant Barcodes (rbcL and matK): Precision of Unsupervised and Supervised Learning Methods towards Accurate Identification. Plants 2021, Vol. 10, Page 2741, 10(12), 2741. https://doi.org/10.3390/PLANTS10122741
  4. Saddhe, A. A., Jamdade, R. A., & Gairola, S. (2020). Recent Advances on Cellular Signaling Paradigm and Salt Stress Responsive Genes in Halophytes. In Handbook of Halophytes (pp. 1–26). Cham: Springer International Publishing. https://doi.org/10.1007/978-3-030-17854-3_111-1
  5. Mosa, K. A., Gairola, S., Jamdade, R., El-Keblawy, A., Al Shaer, K. I., Al Harthi, E. K., … Mahmoud, T. (2019). The Promise of Molecular and Genomic Techniques for Biodiversity Research and DNA Barcoding of the Arabian Peninsula Flora. Frontiers in Plant Science, 9, 1929. https://doi.org/10.3389/fpls.2018.01929
  6. Jamdade, R. A., Mahmoud, T., & Gairola, S. (2019). Prospects of genomic resources available at the global databases for the flora of United Arab Emirates. 3 Biotech, 9(9), 333. https://doi.org/10.1007/s13205-019-1855-9.
  7. Patil, T. S., Jamdade, R. A., Patil, S. M., Govindwar, S. P., & Muley, D. V. (2018). DNA barcode-based delineation of freshwater fishes from northern Western Ghats of India, one of the world’s biodiversity hotspots. Biodiversity and Conservation. https://doi.org/10.1007/s10531-018-1604-0
  8. Saddhe, A. A., Jamdade, R. A., & Kumar, K. (2017). Evaluation of multilocus marker efficacy for delineating mangrove species of West Coast India. PLoS ONE, 12(8). https://doi.org/10.1371/journal.pone.0183245
  9. Saddhe, A. A., Jamdade, R. A., & Kumar, K. (2016). Assessment of mangroves from Goa, west coast India using DNA barcode. SpringerPlus, 5(1). https://doi.org/10.1186/s40064-016-3191-4
  10. Khedkar, G. D., Jamdade, R., Kalyankar, A., Tiknaik, A., Ron, T. B., & Haymer, D. (2014). Genetic fragmentation in India’s third longest river system, the Narmada. SpringerPlus, 3(1). https://doi.org/10.1186/2193-1801-3-385
  11. Khedkar, G. D., Jamdade, R., Naik, S., David, L., & Haymer, D. (2014). DNA barcodes for the FIshes of the Narmada, one of India’s longest rivers. PLoS ONE, 9(7). https://doi.org/10.1371/journal.pone.0101460


GENBANK PUBLICATIONS

Animal Sequences
  • DNA barcoding of fishes from Narmada riverine system, one of the longest river from India, published in Plos One, 2014 (319 sequences: JX983210-JX983514, KF214293-KF214306)
  • Genetic fragmentation in India's third longest river system, the Narmada determined by D-loop gene analysis of Catla catla and Mastacembalus armatus. Published in SpringerPlus, 2014 (102 sequences: KF468009-KF468110)
  • DNA barcoding of ornamental fishes that could help Ornamental Fish trading in changing the regime of Indian Biodiversity Act (106 sequences: JQ667488-JQ667593)
  • Genetic Diversity and Population Structure of Giant Tiger Prawn Penaeus monodon from East Coast of India by Mitochondrial D-Loop (81 sequences: JQ863127-JQ863216)
  • DNA barcoding of Formicidan ants (75 sequences: KC685075-KC685001) 
  • Freshwater sponge barcoding (4 sequences: KC860779-KC860775)
Plant Sequences

PRESENTATIONS

  1. Eighth International Barcode of Life Conference, Trondheim, Norway (2019). "Evaluation of Arabian Vascular Plant Barcodes (rbcL and matK): Precision of Unsupervised and Supervised Learning."
  2. National Conference on Recent Advances and Applications of Taxonomy in Life Sciences (2015). "Identification of Human Pathogenic Fungi through DNA Sequencing."
  3. National Conference on Innovative Ideas & Research in Life Science for Sustainable Development (2015). "Elemental composition of freshwater sponge using Atomic Absorption Spectroscopy from Satara region."
  4. Fourth International Conference on Insect Science (2013). "DNA Barcoding of Formicidean ants from Western Ghats of Maharashtra."
  5. International Conference on Biodiversity and its Conservation (2011). Modern College of Arts, Science & Commerce Pune. "Freshwater Sponge fauna of Western Ghats."
  6. International workshop on Aquatic Bioresource Biotechnology & Parasite Diversity (2009). Department of Zoology, Allahabad Central University, Allahabad. "Protocol for Easy and Economic DNA Isolation."

RESOURCE PERSON

  1. International Conference on Recent Trends in Basic and Applied Sciences. R.B. Narayanrao Borawake College, Shrirampur, MH, India. (14th May 2022). Artificial Intelligence for accurate species identification.
  2. International Conference on Advances in Science and Technology. Rajashri chhatrapati Shahu College, Kolhapur, MH, India (9th March 2022). Artificial Intelligence for accurate species identification.
  3. International Webinar on 'Taxonomy and Forensic Entomology. Sant Gadge Baba Amravati University, Amravati, MH, India (31st May 2020). Artificial Intelligence for accurate species identification.
  4. Two days hands-on workshop on Bioinformatics. Yashavantrao Chavan Institute of Science, Satara, MH, India (12th - 13th Nov. 2018). 
  5. Two days hands-on workshop on Bioinformatics. Yashavantrao Chavan Institute of Science,Satara, MH, India (18th - 19th Aug. 2017). 
  6. Yashavantrao Chavan Institute of Science,Satara, MH, India (22nd July 2016). An overview to Ubuntu Operating System.
    WORKSHOPS

      1. National workshop on Integrative Taxonomy of Freshwater Fishes (2015), organized by Kerala University of Fisheries and Oceanic Studies, Kochi, Kerala, India.
      2. Workshop on Revised Syllabus of Zoology (B.Sc III Practicals) (2015), organized by Lal Bahadur Shastri College of Arts, Science & Commerce, Satara.
      3. Students and Teachers Workshop on Concepts & Research in Bioinformatics (2015), organized by Yashavantrao Chavan Institute of Science, Satara.
      4. Workshop on Basic PERL for Life Sciences (2014), organized by BIoSakashta, NCL innovation park, Pune.
      5. Second Strategic meeting and workshop on DNA Barcoding Initative (2011), organized by Paul Hebert Centre for DNA Barcoding and Biodiversity Studies, Aurangabad.
      6. Operation & Maintenance of Laboratory Instruments (2011), organized by Government autonomous post graduate college Chhindwara, Madhya Pradesh.
      7. First strategic meeting and workshop on DNA Barcoding Initiative (2010), organized by Paul Hebert Centre for DNA Barcoding and Biodiversity Studies, Aurangabad.

      REFERENCES
      • Dr. Kareem Mosa: Associate Professor, University of Sharjah (UAE). Email:kmosa@sharjah.ac.ae
      • Dr. Kundan Kumar: Associate Professor, Department of Biological Sciences. BITS Pilani K K Birla Goa Campus. Email: kundan@goa.bits-pilani.ac.in.
       

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